Plaque Formation by Arboviruses

Plaque formation by bluetongue virus.

The cytopathic changes produced by bluetongue virus were first observed by Haig, McKercher & Alexander (1956) in monolayer roller tube cultures of primary lamb kidney ce ll s. Subsequently, Fernandes (1959) described the growth and cytopathic effects of a virulent as well as a homologous egg-adapted strain at the thirtysixth passage level, in cultures of human amnion (Fernandes), Chang liver, c...

Immunological overlap and identification of group A arboviruses by a gradient plate plaque technique.

A semi-quantitative, gradient plate, plaque-neutralization technique was developed for studying immunological overlap and identification of Group A arboviruses. Two principles of the technique are: (i) a concentration gradient of antibody is provided that continues into a control area without antibody, and (ii) neutralization is measured by inhibition of plaque formation in agar-cell suspension...

Plaque formation by Chlamydia in L cells.

Chlamydiae were found capable of producing plaques in several cell lines. Mouse fibroblast cells, L-929, proved the most sensitive to infection and yielded plaques of the highest clarity. Assay of chlamydial infectivity by plaque titration was at least as sensitive as egg ld(50) determination. Among chlamydial isolates of avian, mammalian, and human origin, only slow-growing trachoma-inclusion-...

Plaque formation by 55 rhinovirus serotypes.

Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced p...

The Formation of Plaques by African Horse Sickness Viruses and Factors Affecting Plaque Size